The gp test in STEPS

1.0 Dilute reference and investigated samples.

  • Dispense 100μl of prepared dilutions of investigated and reference samples per well.
  • Incubate 90 minutes at 37 °C, shake gently.

2.0 Prepare biotinylated anti-Rabies antibody.

  • Wash plates 2x time.
  • Dispense 100μl of biotinylated anti-Rabies antibody diluted to 1/100 to all wells.
  • Incubate 30 minutes at 37°C, shake gently.

3.0 Prepare streptavidin peroxidase conjugate.

  • Wash plates 4x time.
  •  Dispense 100μl of streptavidin peroxidase conjugate diluted to 1/100 to all wells.
  • Incubate 30 minutes at 37°C, shake gently.

4.0 Wash plates 4x time.

  •  Dispense 100μl of TMB Substrate to all wells.
  •  Incubate 15-20 minutes at room temperature (18 – 25°C), shake gently.

5.0 Stop reaction with 50μl of Stop solution.

  • Read absorbance (OD) at 450 nm.
  • Check validation criteria.
  • Interpret  result.

Please, do not hesitate to contact us for further details or package insert.

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