It is very easy to use the BioPro RABIES ELISA Ab kit:

1. Dilute samples & controls

• incubate overnight (18-24 hours) at 2-8ºC

2. Wash 6 times, add Biotinylated anti-Rabies antibody

• incubate 30 minutes at 37ºC

3. Wash 4 times, add Streptaviding peroxidase conjugate

• incubate 30 minutes at 37ºC

4. Wash 4 times, add TMB substrate

• incubate 15-30 minutes at room temperature

5. Add Stop solution, read the plate at 450nm

• calculate results

• interpret results


The procedure in details is described in package insert.

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